Announcement PDF BPH GLOBAL LTD ACN 009 104 330 1 April 2025 Company Announcements Pla7orm Australian Securi9es Exchange Encouraging gold and silver assays from Phase 2 seaweed cul7va7on Highlights • Assays from sample 1 of Phase 2 Seaweed grown using seaweed culGvated in the polluted, brackish waters in Johor, Malaysia show significantly higher mineral content compared to seaweed culGvated in clean, prisGne water (Phase 1) • Gold Assays up to 123.16 mg/kg – over eight Gmes the amount assayed in Phase 1 • Silver Assays up to 80.58mg/kg – over six Gmes the amount assayed in Phase 1 • Further test work to be conducted on addiGonal batches of Phase 2 seaweed The Board of BPH Global Ltd (ASX: BP8) (Company), is pleased to announce the latest laboratory assay results from its ongoing R&D program conducted by Temasek Innova9on Holdings Pte Ltd (TPIH), an opera9ng company of Temasek Polytechnic in Singapore, in collabora9on with the Company’s Singapore-based R&D consultant Gaia Mariculture Pte Ltd (Gaia Mariculture). Managing Director MaWhew Leonard said: “The significantly higher concentra9ons of minerals, including gold and silver, found in the first assay results from Phase 2 of our R&D program represent an exci9ng milestone for the Company. These ini9al findings support our hypothesis that seaweed cul9vated in polluted seawaters contains higher mineral concentra9ons compared to seaweed grown in clean, pris9ne waters. We look forward to further inves9ga9ng this hypothesis with addi9onal seaweed batches in Phase 2 of the R&D program.” Phase 2 Work Plan and comparison to Phase 1 Work Plan results As detailed in the Company’s announcements on 21 February 2025 and 7 March 2025, Phase 1 focused on the cul9va9on of the seaweed species Sesuvium Portucalastrum in a controlled laboratory se^ng. The process, ini9ated in early December 2024 at Gaia Mariculture’s Singapore facility, used clean, filtered seawater sourced from Sentosa Island—free from industrial or urban pollu9on and sediment. Phase 2 is based on Sesuvium Portucalastrum seaweed harvested from brackish waters of Johor, Malaysia that are proximate to shipping lanes and industrial ac9vity. The aim of Phase 2 is to test the hypothesis that seaweed absorbs higher mineral concentra9ons in dirty, polluted waters compared to seaweed grown in pris9ne waters due to the “sponge” effect. It is the Company’s view that seaweed has the poten9al to act like a sponge for base and precious metals in seawater. Following the assay results from Phase 1, TPIH conducted assays on Batch 1 of Phase 2 to measure its mineral content. The tes9ng was expanded to include a broader range of minerals. As with the Phase 1 batches, the samples in Batch 1 of Phase 2 were processed with concentrated nitric acid and hydrogen peroxide, filtered, and then diluted for analysis using Induc9vely Coupled Plasma–Mass Spectrometry (ICP-MS). The results are presented in Tables 1 and 2 below: Table 1: Assay results of samples from Phase 1 (Batch 1 and 2) and Phase 2 (Batch 1) u?lising ICP-MS Phase 2 Metal Phase 1 Metal Content (mg/kg) Content (mg/kg) Metal Batch 1 Batch 2 Batch 1 123.16 Au 14.85 Not tested 80.58 Ag 2.76 12.67 7.59 Co 13.68 0.25 Ni 0.80 0.89 8.57 17.24 As 0.53 0.52 84.33 Cu 10.88 Not tested Cd 0.31 0.00 8.89 9.95 Pb 0.93 0.61 Table 1: Addi?onal tested metal content of Phase 2 Batch 1 u?lising ICP-MS Li Phase 2 Metal Content (mg/kg) 15.08 Be Mg Al 9.98 4202.13 0 K Ca 10625.60 2162.18 V Cr Mn 29.11 16.10 36.45 Fe 82.11 Cu Zn Ga 84.33 20.03 9.25 Rb Sr 10.26 45.08 Mo 19.64 Metal In Cs Ba 9.54 6.73 9.16 Ti Pb Bi 13.53 9.95 11.58 Phase 1 R&D ObjecGves and Findings The primary objec9ve of Phase 2 is to assess the mineral absorp9on capabili9es of Sesuvium portucalastrum seaweed grown in polluted waters near shipping lanes and onshore industrial sites. Significant assay results included: • Gold Assays up to 123.16 mg/kg – over eight 9mes the amount assayed in Batch 1 of Phase 1. • Silver Assays up to 80.58mg/kg – over six 9mes the amount assayed in Batch 2 of Phase 1. • Copper Assays up to 84.33mg/kg – almost eight 9mes the amount assayed in Batch 1 of Phase 1. The analysis of assay results from Batch 1 of Phase 2 supports the following hypotheses: (i) Sesuvium portucalastrum appears to be an effec9ve absorber of minerals, including gold, silver, and copper; and (ii) seaweed absorbs higher concentra9ons of minerals when cul9vated in polluted waters compared to those grown in pris9ne waters. Next Steps Building on the ini9al assays of Batch 1, TPIH will conduct further assays on addi9onal batches from the Phase 2 harvest of Sesuvium portucalastrum sourced from Johor, Malaysia. This will allow the Company to further test its hypothesis that seaweed absorbs higher concentra9ons of minerals in polluted waters than in pris9ne waters, due to the 'sponge' effect. During Phase 1, TPIH commenced work on techniques for the extrac9on of minerals from Sesuvium portucalastrum seaweed. This research work will con9nue throughout Phase 2 of the Company’s R&D program. Further updates The Company will provide further updates regarding the Phase 2 cul9va9on and assays cycle and associated research and development. This announcement has been authorised by the Board of Directors. For further informa9on, please visit our website at www.bp8global.com or contact: Mr Paul Stephenson BPH Global Limited Chairman E: pauls@bp8global.com Appendix 1: Disclosure Table providing key details of assay results AcGvity DescripGon CulGvaGon & Harvest Site: The biomass samples of Sesuvium portucalastrum seaweed were harvested from the Straits Of Johor coastline, nearshore. CulGvaGon Date: The harvested seaweed was naturally occurring in the area (Johor, Malaysia) Harvest Date: 14:00pm on 24 March 2025 Post harvest storage pending transport to TPIH: The samples were stored in 3 separate large low density polyethylene bags, making up a total of more than 5kg in weight. Mode and duraGon of transport from harvest site to TPIH: The bagged samples were transported by vehicle to the tes9ng facility at TPIH and arrived at 16:58pm on 24 March 2025.. Moisture content in seaweed on arrival at TPIH: 86.0% Processing method and Assay equipment: The seaweeds were les to air-dry overnight (~16 hours) to remove surface water. A small sample was taken for moisture and metal content determina9on. The remainder, some of which will be sampled as further batches for assay tes9ng, (approximately 3kg) were then mass grinded and laid out on pans for oven drying at 70°C for 1 week (25 Mar-1 Apr 2025). Seaweeds which were not processed immediately were stored in the cold room at 4 degrees Celsius. The samples from Batch 1 that were allocated to assaying for metal content were selected on a random basis. These selected samples were digested using concentrated nitric acid and hydrogen peroxide. Between 30-35g of these samples, randomly selected, were dried, and subsequently one (1)g of the dried samples, again randomly selected, were used for metal quan9fica9on. The one (1)g of the dried samples were then filtered and diluted to facilitate metal determina9on using Induc9vely Coupled Plasma– Mass Spectrometry (ICP-MS). The one (1)g of the dried seaweed samples were digested using 15 ml of concentrated nitric acid at 80°C for two (2) hours. Five (5) ml of hydrogen peroxide (15 %) was then added, and the mixture was maintained at 80°C for another two (2) hours. The mixture was then cooled to room temperature and filtered. The filtrate was diluted to enable metal determina9on using Induc9vely Coupled Plasma – Mass Spectrometry (ICP-MS). Diges9on of samples using nitric acid and hydrogen peroxide is a commonly used procedure for metal extrac9on from seaweed biomasses. ICP-MP is the standard instrumenta9on used for metal detec9on and quan9fica9on. Processing date: 25 March 2025