Announcement PDF BPH GLOBAL LTD ACN 009 104 330 30 July 2025 Company Announcements Pla6orm Australian Securi:es Exchange Final Assay Results Confirm Metal Uptake Poten8al of Seaweed Species Sesuvium portulacastrum Highlights • Final assay results confirm Sesuvium portulacastrum is a hyperaccumulator of key metals including gold, silver, copper, and lithium, with concentraJons up to 81.32 mg/kg of dry biomass. • R&D program confirms metal uptake is significantly higher in seaweed grown in polluted/brackish water compared to clean marine environments. • ARempts to extract specific metals using convenJonal chemical methods were unsuccessful due to the complex mulJ-metal profile of the biomass. • Biomass now classified as “bio-ore”, with the next phase of research to focus on biological (microbial) extracJon techniques. • New six-month R&D program under negoJaJon with TPIH, involving a specialist biology team to lead development of selecJve extracJon processes. • Seaweed species found to be resilient and widely adaptable, supporJng future potenJal for scalable deployment across diverse marine environments. The Board of BPH Global Ltd (ASX: BP8) (Company), is pleased to announce the successful comple:on of the Company’s ini:al six-month research and development (R&D) program into the iden:fica:on and poten:al extrac:on of precious and rare earth minerals from seaweed. The program was conducted by Temasek InnovaJon Holdings Pte Ltd (TPIH), an opera:ng company of Temasek Polytechnic in Singapore, in collabora:on with the Company’s Singapore-based R&D consultant Gaia Mariculture Pte Ltd (Gaia Mariculture). Managing Director MaShew Leonard said: “The final assay results from Phase 2 confirm that Sesuvium portulacastrum is a hyperaccumulator of several high-value metals, including gold, silver, copper and lithium. These metals were consistently detected in moderately high to high concentra:ons across four separate samplings, with levels ranging between 5.23 and 81.32 milligrams per kilogram of dry biomass. This is a significant milestone for our R&D program and supports our vision of scalable seaweed cul:va:on in targeted environments for the poten:al extrac:on of precious metals. The findings also confirm the robust adaptability of Sesuvium, capable of thriving in a broad range of salinity and habitat condi:ons, which bodes well for future deployment across diverse coastal regions.” Summary of Final R&D Findings The final report delivered by TPIH provides a comprehensive overview of the mineral uptake capacity of Sesuvium portulacastrum, a halophy:c seaweed species, in polluted marine environments. Key findings include: • Detec:on of 28 metal species in the weed :ssue, based on available analy:cal reagent standards. • Notably high concentra:ons of gold, silver, copper and lithium, sugges:ng the plant acts as a hyperaccumulator for these and other metals. • Concentra:on range for the four metals of interest averaged between 5.23 and 81.32 mg/kg dry biomass. • Confirma:on that Sesuvium is resilient and adaptable, capable of surviving in salinity condi:ons ranging from brackish inland waters to full-strength marine environments. • Poten:al applicability across the Indonesian archipelago and similar coastal regions, where the species’ environmental tolerance could be leveraged for scalable deployment. Background and R&D Program Summary As detailed in the Company’s announcements dated 21 February 2025, 7 March 2025 and 1 April 2025, the R&D program was structured in two key phases: • Phase 1 involved cul:va:ng Sesuvium portulacastrum under laboratory condi:ons using clean, filtered seawater sourced from Sentosa Island, Singapore, to establish baseline mineral absorp:on. Phase 2 assessed seaweed samples collected from brackish, industrially impacted waters in Johor, Malaysia, to test the hypothesis that polluted or mineral-rich waters enhance metal uptake in the seaweed through a “sponge” effect. The combined assay results from Phase 2 validate the Company’s hypothesis, demonstra:ng meaningful increases in metal concentra:on levels in seaweed samples grown in polluted condi:ons when compared to the baseline established under clean water condi:ons in Phase 1. Challenges in Mineral SeparaJon and Path Forward While metal uptake by the biomass has been confirmed, the second objec:ve - the separa:on and extrac:on of specific metals from the dissolved biomass—has proven more complex. Using conven:onal chemical reagent-based techniques to isolate and extract gold from the aqueous solu:on produced by dissolved seaweed was unsuccessful. Although a metallic precipitate was formed, it consisted of a mixed metal compound rather than purified gold. The presence of mul:ple interfering metals within the biomass appears to limit the effec:veness of tradi:onal chemical extrac:on methods. Temasek’s report notes: “Biomasses typically contain many more metal species than mined earthen ores, and the abundance of metal types interferes with conven:onal chemical separa:on techniques. While we managed to precipitate a lump of metallic compound, it was not pure gold, but a mixed metal matrix.” As a result, the Company and TPIH will now treat the seaweed biomass as “bio-ore”, with plans to shig focus to biological extrac:on techniques. Microorganisms, with their narrow-range affini:es for specific metals, may offer a more targeted and selec:ve extrac:on mechanism than chemical methods. To support this shig, a new six-month R&D program is being nego:ated with TPIH, with research to be conducted by a new biology-focused team possessing the requisite exper:se in biometallurgy, microbial metal uptake, and biologically-assisted leaching. This next phase aims to test the feasibility of biological methodologies for the isola:on and refinement of selected metals from the seaweedderived solu:on. Next Steps The Company’s key forward ini:a:ves include: • Finalisa:on of a new six-month R&D agreement with TPIH focused on biological extrac:on; • Deployment of a new research team within TPIH with appropriate biological and microbiological exper:se; • Ini:al tes:ng of microbial-assisted extrac:on processes on Sesuvium biomass samples; • Explora:on of poten:al partnerships or commercial interest in “bio-ore” solu:ons for metal recovery; • Con:nua:on of seaweed sourcing from strategic loca:ons with known mineral-rich marine waters. Further updates The Company will con:nue to update shareholders regarding the Ini:al tes:ng of microbial-assisted extrac:on processes on Sesuvium biomass samples and associated research and development. This announcement has been approved for release by the Board of Directors. - END For further informa=on, please visit our website at www.bp8global.com or contact: Mr Paul Stephenson BPH Global Limited Chairman E: pauls@bp8global.com Appendix 1: Disclosure Table providing key details of assay results of Phase Two AcJvity DescripJon CulJvaJon & Harvest Site: The biomass samples of Sesuvium portucalastrum seaweed were harvested from the Straits Of Johor coastline, nearshore. CulJvaJon Date: The harvested seaweed was naturally occurring in the area (Johor, Malaysia) Harvest Date: 10:30am on 24 March 2025 Post harvest storage pending transport to TPIH: The samples were stored in 3 separate large low density polyethylene bags, making up a total of more than 5kg in weight. Mode and duraJon of transport from harvest site to TPIH: The bagged samples were transported by vehicle to the tes:ng facility at TPIH and arrived at 14:00pm on 24 March 2025.. Moisture content in seaweed on arrival at TPIH: 85.4% Processing method and Assay equipment: The seaweeds were leg to air-dry overnight (~16 hours) to remove surface water. A small sample was taken for moisture and metal content determina:on. The remainder, some of which were sampled for assay tes:ng, (approximately 3kg) were then mass grinded and laid out on pans for oven drying at 70°C for 1 week (25 Mar-1 Apr 2025). Seaweeds which were not processed immediately were stored in the cold room at 4 degrees Celsius. The samples from Phase Two that were allocated to assaying for metal content were selected on a random basis. These selected samples were digested using concentrated nitric acid and hydrogen peroxide. Between 30-35g of these samples, randomly selected, were dried, and subsequently one (1)g of the dried samples, again randomly selected, were used for metal quan:fica:on. The one (1)g of the dried samples were then filtered and diluted to facilitate metal determina:on using Induc:vely Coupled Plasma– Mass Spectrometry (ICP-MS). The one (1)g of the dried seaweed samples were digested using 15 ml of concentrated nitric acid at 80°C for two (2) hours. Five (5) ml of hydrogen peroxide (15 %) was then added, and the mixture was maintained at 80°C for another two (2) hours. The mixture was then cooled to room temperature and filtered. The filtrate was diluted to enable metal determina:on using Induc:vely Coupled Plasma – Mass Spectrometry (ICP-MS). Diges:on of samples using nitric acid and hydrogen peroxide is a commonly used procedure for metal extrac:on from seaweed biomasses. ICP-MP is the standard instrumenta:on used for metal detec:on and quan:fica:on. IniJal Processing date: 25 March 2025